Figure 2.

Differential mast cell activation by various antigens. (A) Effects of antigen-specific IgE occupancy on mast cell surface FcεRIs. (B) Effects of antigen-binding durations. (C) Effects of affinity between IgE and its cognate antigens. The antigen concentration is adjusted so that the phosphorylation levels of FcεRIβ and γ chains are similar. Abundant low-affinity antigens induce few and large clusters, while a small amount of high-affinity antigens induce many small clusters. (D) Effects of spacing between antigen epitopes. (E) Effects of antigen valency and/or IgE clonality. (F) Effects of affinity of different clones against the same antigen.