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. 2021 Jul 7;22(14):7327. doi: 10.3390/ijms22147327

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Apoptosis study. Flow cytometry from healthy (AG03257, AG03258, AG03512, and AG06299) and HGPS (AG03198, AG03199, AG03513, and AG06917) human skin fibroblasts treated with and without NAC. (A) Representative FAC profile showing the 7-ADD in front of Annexin V staining using FlowJo software V8.6 (Treestar, OR, USA) from HGPS skin fibroblasts treated with or without NAC. (B) Histograms represent the percentage of living cells with the different treatments. Bars are means ± SEM from three independent experiments. * p < 0.05 was considered statistically significant versus healthy cell lines. # p < 0.05 was considered statistically significant versus treatments. STS = staurosporine; Tg = thapsigargin; H₂O₂ = hydrogen peroxide; DMSO = dimethyl sulfoxide.