Table 1.

Total and individual GSL content (µmol·g⁻¹·dw) in leaves of Chiffu, a parent of LP08 and LP21, F1, and eight BrYSP_DH lines of Brassica rapa.

Genotype		Aliphatic							Indolic			Aromatic
		GRA	GAL	SNG	GNA	GBN	GNL	PRO	GBS	4-MOGBS	NGBS	GNT	Total μmol g−1 dw
	Chiffu	ND	ND	ND	0.00 e	0.00 f	0.00 c	0.38 ± 0.00 def	4.00 ± 0.26 a	1.09 ± 0.04 a	3.16 ± 0.52 b	0.14 ± 0.09 de	8.77 ± 0.69 ef
	LP08(♀)	0.11 ± 0.05 b	0.55 ± 0.05 g	0.17 ± 0.00 a	43.40 ± 3.63 b	0.66 ± 0.06 e	0.00c	0.71 ± 0.06 cd	0.40 ± 0.01 d	0.31 ± 0.04 c	0.76 ± 0.06 d	0.47 ± 0.14 bc	47.55 ± 3.66 bc
	LP21(♂)	0.00b	0.89 ± 0.10 def	0.00e	1.82 ± 0.66 d	0.00f	0.27 ± 0.03 a	0.88 ± 0.09 c	2.03 ± 0.02 b	0.63 ± 0.03 b	3.44 ± 0.24 b	0.81 ± 0.09 a	10.76 ± 0.36 e
	F1	0.10 ± 0.01 b	0.85 ± 0.12 ef	0.00e	1.97 ± 0.16 d	1.68 ± 0.14 d	0.34 ± 0.03 a	3.86 ± 0.29 a	0.59 ± 0.02 c	0.64 ± 0.03 b	4.58 ± 0.16 a	0.47 ± 0.05 bc	15.07 ± 0.79 d
High GSL lines	BrYSP_DH005	1.23 ± 0.18 a	3.47 ± 0.18 a	0.05 ± 0.01 cde	35.51 ± 2.84 c	0.63 ± 0.04 e	0.10 ± 0.04 b	0.22 ± 0.03 ef	0.14 ± 0.00 e	0.06 ± 0.00 f	2.03 ± 0.20 c	0.68 ± 0.34 ab	44.12 ± 2.86 c
	BrYSP_DH014	0.07 ± 0.02 b	2.16 ± 0.05 b	0.05 ± 0.01 cde	45.19 ± 2.69 ab	7.13 ± 0.62 a	0.02 ± 0.01 bc	0.06 ± 0.02 f	0.11 ± 0.01 e	0.10 ± 0.01 ef	0.77 ± 0.04 d	0.39 ± 0.04 cd	56.06 ± 3.28 a
	BrYSP_DH016	0.12 ± 0.07 b	0.43 ± 0.05 g	0.11 ± 0.07 b	44.42 ± 1.34 b	1.68 ± 0.07 d	ND	0.57 ± 0.37 cde	0.20 ± 0.01 e	0.16 ± 0.01 d	0.76 ± 0.02 d	0.24 ± 0.03 cde	48.69 ± 0.95 b
	BrYSP_DH017	0.07 ± 0.01 b	0.62 ± 0.17 fg	0.08 ± 0.02 bc	45.66 ± 1.25 ab	2.70 ± 0.09 c	0.10 ± 0.11 b	0.32 ± 0.03 ef	0.19 ± 0.01 e	0.19 ± 0.02 d	0.50 ± 0.02 def	0.20 ± 0.01 cde	50.62 ± 1.51 b
	BrYSP_DH026	0.09 ± 0.02 b	2.05 ± 0.04 b	0.07 ± 0.04 bcd	48.23 ± 1.31 a	5.39 ± 0.25 b	0.03 ± 0.02 bc	0.05 ± 0.00 f	0.08 ± 0.00 e	0.11 ± 0.00 e	0.57 ± 0.05 de	0.39 ± 0.03 cd	57.04 ± 1.54 a
Low GSL lines	BrYSP_DH009	0.04 ± 0.03 b	1.16 ± 0.10 d	0.00 ± 0.00 e	0.25 ± 0.05 d	0.26 ± 0.07 ef	0.09 ± 0.03 b	0.82 ± 0.26 c	0.12 ± 0.02 e	0.10 ± 0.01 ef	0.33 ± 0.03 ef	0.08 ± 0.03 e	3.26 ± 0.62 g
	BrYSP_DH059	0.06 ± 0.01 b	1.44 ± 0.19 c	0.01 ± 0.01 de	0.32 ± 0.06 d	0.52 ± 0.08 ef	0.30 ± 0.03 a	1.97 ± 0.23 b	0.10 ± 0.02 e	0.07 ± 0.01 ef	0.38 ± 0.03 def	0.15 ± 0.02 de	5.31 ± 0.67 fg
	BrYSP_DH061	ND	1.13 ± 0.26 de	0.01 ± 0.01 de	2.35 ± 0.81 d	1.70 ± 0.64 d	0.00 ± 0.00 c	0.05 ± 0.01 f	0.05 ± 0.01 e	0.01 ± 0.00 g	0.11 ± 0.02 f	0.23 ± 0.07 cde	5.63 ± 1.76 fg
ANOVA	Sum of square	3.852	29.573	0.091	16,645	165.68	0.525	39.6	46.36	3.601	72.88	1.669	16,984
	Mean sum of square	0.350	2.688	0.008	1513.2	15.061	0.047	3.6	4.215	0.327	6.626	0.152	1544
	F value	68.22	103.25	9.09	348.84	132.28	22.81	80.11	471.38	494.16	129.53	7.5	286.69
	p	****	****	****	****	****	****	****	****	****	****	****	****

GRA, Glucoraphanin; GAL, Glucoalyssin; SNG, Sinigrin; GNA, Gluconapin; GBN, Glucobrassicanapin; GNL, Gluconapoleiferin; PRO, Progoitrin; GBS, Glucobrassicin; 4-MOGBS, 4-Methoxyglucobrassicin; NGBS, Neoglucobrassicin; GNT, Gluconasturtiin; ND, Not detected. Different letters indicate significant difference between the genotypes under Duncan’s test (p ≤ 0.05) for three individual biological replicates. F-test are significant at **** p ≤ 0.0001 or Nonsignificant, respectively.