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. 2021 Jun 29;12(7):989. doi: 10.3390/genes12070989

Figure 4.

Figure 4

SF challenge test on THP1 cells—the test was carried out to assess MMPs modulation in inflamed THP1. These cells were treated with 10% SFs (of culture media) of progressive OA grades for 48 hrs in order to induce inflammation. Inflammation induction test—(A) NO release after 48 hrs of SF treatment on THP1 cells; NO significance, revealed during inter-grade comparison is showed in table a1. MMP-specific response of the inflamed cells—(B) MMP-1: the highest rise in the expression in the cells treated with SFs from KL grade-II; (C) MMP-13: a grade-wise elevation was seen in MMP-13 expression; the maximum up-regulation was found in the cells treated with SFs from KL grade-IV; (D) VEGF: the highest estimation of VEGF was in the cells treated with KL grade-II SFs that was followed by a grade-wise decline in the expression; the lowest expression was seen in the cells treated with SFs from KL grade-I. Inter-grade difference for MMP-1, MMP-13 and VEGF−1 on the cells is showed in tables b1, c1 and d1, respectively. * p < 0.05, ** p < 0.01, *** p < 0.001.