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. 2021 Jul 23;20:150. doi: 10.1186/s12933-021-01346-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — A EMPA reduced significantly the DOXO -induced ferroptosis in cardiac tissues, determined by Mitochondrial lipid peroxidation, measured using MitoPeDPP (ratio to sham). EMPA reduced significantly the DOXO -induced MDA content in cytosolic (B) and mitochondrial fraction (C) of cardiac tissues (ratio to sham). D EMPA reduced significantly the xanthine oxidase content in cardiac tissues during treatment with DOXO (mU/mg of protein). E EMPA exerts anti-inflammatory effects on the liver, heart and kidney of mice treated with DOXO. F EMPA reduces pro-inflammatory markers MyD88 and NLRP3 in cardiac tissue during DOXO treatments. For both, we quantified the expression of interleukin 1-β, interleukin 6 and interleukin 8 (pg/mg of protein) in heart, liver and left kidney lysates of mice untreated (Sham) or treated with EMPA, DOXO or DOXO/EMPA for 7 days (n = 6 for each group); in heart tissues only, we quantified the expression of MyD88 and NLRP3 through mouse ELISA kits (pg of marker/mg of protein). One-way ANOVA. Values are expressed ± SD.*** P < 0.001; **P < 0.01; *P < 0.05; ns: not significant