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. 2021 Jul 14;22(14):7541. doi: 10.3390/ijms22147541

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Validation of Sm-miR408 target genes. (A) Relative expression levels of the Sm-miR408 putative target genes in amiR408 lines compared to control lines. (B) Relative expression levels of the Sm-miR408 putative target genes under normal and copper starvation conditions. (C) Sm-miR408 cleavage site in SmLAC3 mRNA validated by 5’ RLM-RACE. For RT-qPCR, all data represent the means of three biological replicates, with error bars indicating SD. Significant differences were determined by Student’s t-test (** p < 0.01).