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. 2021 Jul 14;19(7):394. doi: 10.3390/md19070394

Figure 1.

Figure 1

Different molecular masses of AKLs found in the eggs of Aplysia kurodai. AKL mixtures obtained from the melibiose column were combined and separated by using Sephacryl S-200 gel filtration column (black triangles show the polypeptides indicating AKLs). AKL-40: 40 kDa species (used in this study), AKL: 32/30 kDa species, and AKL-2: 16 kDa species. M: standard markers of Phosphorylase b (94 kDa), serum albumin (66 kDa), ovalbumin (42 kDa), carbonic anhydrase (30 kDa), trypsin inhibitor (21 kDa), and lysozyme (14 kDa). All samples are separated in the reducing condition and 15% polyacrylamide gels are used except AKL-2, which was separated by a 12% gel.