Fig. 6.

In vitro angiogenic property of the Co (0, 1, 2.5, 5) scaffolds. A Micrographs of HUVECs cultured in Co (0, 1, 2.5, 5) extracts for 4, 6, and 16 h (scale bar = 100 µm). B, C Statistical analysis of the branches number and total branching length. D Western blot analysis of HIF-1α and VEGF proteins in rBMSCs cultured in Co (0, 1, 2.5, 5) extracts for 7 days and their quantitative analysis (E). The data are expressed as the mean ± SD. *p < 0.05 and **p < 0.01, comparison with the Co0 group; ^#p < 0.05, comparison among the Co (1, 2.5, 5) groups