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. 2021 Jul 24;20:70. doi: 10.1186/s12938-021-00907-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — In vitro osteogenic properties of the Co (0, 1, 2.5, 5) scaffolds. A Micrographs of ALP staining at days 7 and 14 and B alizarin red staining at day 21 are shown (scale bars = 100 µm). The quantitative analysis of ALP activity (C) and calcium content (D) are shown. The group in which rBMSCs were cultured in osteogenic medium (OM) was set as the positive control group. The data are expressed as the mean ± SD. *p < 0.05 and **p < 0.01, comparison with the OM group; ^#p < 0.05, comparison among the Co (0, 1, 2.5, 5) groups. E Western blot analysis of osteogenic markers (ALP, BMP-2, OCN and RUNX2) and their quantitative analysis (F) in rBMSCs stimulated by the Co (0, 1, 2.5, 5) extracts for 14 days. The data are expressed as the mean ± SD. *p < 0.05 and **p < 0.01, comparison with the Co0 group; ^#p < 0.05, comparison among the Co (1, 2.5, 5) groups