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. 2021 Jul 6;10(7):1709. doi: 10.3390/cells10071709

Figure 6.

Figure 6

miRNA-132/212 deletion altered AChE and mAChR-M1 protein expression. Protein expression levels of AChE, mAChR-M1, nAChR-α7 and MeCP2 were determined by Western blot analysis from total cell lysates of female mouse bilateral hippocampi. The left columns (A–D and numbered with 1 in each case) depict representative immunoblots for the proteins of interest and GAPDH loading controls. The right columns (A–D and numbered with 2 in each case) depicts aligned scatter plots representing the protein levels of the respective proteins of interest, normalized to their respective GAPDH loading control protein levels, for lysates from miRNA 132/212−/− and WT control mice. (A) Lysates from miRNA 132/212−/− mice exhibited significantly higher AChE expression levels, as compared to their respective WT controls. (B) In contrast, lysates obtained from miRNA 132/212−/− mice showed significantly lower mAChR-M1 expression levels, as compared to WT controls. However, samples from miRNA 132/212−/− and WT control mice did not differ significantly regarding (C) nAChR-α7 and (D) MeCP2 protein expression. Data is expressed as mean ± SEM. n = 5 per group. AChE: Acetylcholinesterase, nAChR-α7: alpha-7 nicotinic receptor, mAChR-M1: muscarinic acetylcholine receptor M1, MeCP2: methyl-CpG binding protein 2. p < 0.05 was considered significant, * p < 0.05, ns: no statistical significance.