Figure 4.

Biovolumes (a,c,e) and thickness (b,d,f) of S. aureus and P. aeruginosa single-species biofilms, and of dual-species biofilms (S. aureus + P. aeruginosa) on PLA and CS-coated PLA surfaces. These parameters were obtained from confocal image series using the COMSTAT2 tool associated with the ImageJ software. The means ± standard deviations for three independent experiments are illustrated. Statistical analysis was performed using one-way analysis of variance (ANOVA) and significant differences were represented for p-values < 0.05 by *, ○, □, and Δ when compared with PLA, β-CS, CS1, and CS2, respectively.