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. 2021 Jul 19;22(14):7695. doi: 10.3390/ijms22147695

Figure 5.

Figure 5

Treatment with anti-IL-17 antibody reduced the pro-inflammatory cytokine production in the spleens of STAT1-/- mice. Mice were treated as in Figure 3A. In total, 1 × 105 spleen cells were obtained at day 20 after AOM/DSS treatment and were stimulated with α-CD3 for 72 h to analyze the expressions of (A) IFN-γ, (B) IL-6, (C) IL-17A, (D) IL-10, and (E) TNF-α cytokines using the Mouse Th1/Th2/Th17 CBA Kit (BD®). Total spleen cells were analyzed by flow cytometry. Frequencies of total (F) CD4+ cells, (G) CD8+ cells, and (H) CD4+Foxp3+CD25+ T cells of tumor-bearing WT iso, STAT1-/- iso or WT or STAT1-/- mice treated with anti-IL-17 neutralizing antibody. Data shown are the mean ± SEM of three or four individual mice per group and are representative of two individual experiments with similar results. * p < 0.05, ** p < 0.01, ***p < 0.001.