Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 23;19:317. doi: 10.1186/s12967-021-02995-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 5 — Constipation induced gut microbiota dysbiosis aggravated Treg/Th17 and Treg17/Teff17 imbalance in CNS of EAE mice. Lymphocytes were harvested from the brains and spinal cords of all mice on 25^st day p.i. and subsequently analyzed by flow cytometry. Mononuclear cells were first gated for lymphocytes followed by the gating of CD4⁺ T cells, and then analyzed for the percentage of Treg (CD4⁺Foxp3⁺) cells, Th17 (CD4⁺IL-17A⁺) cells, Treg17 (CD4⁺IL-17A⁺ Foxp3⁺) cells and Teff17 (CD4⁺IL-17A⁺ Foxp3⁻) cells. Representative flow data showed percentage of Treg, Th17, Treg17 and Teff17 cells in the brains and spinal cords gated on CD4 positive cells of three groups (a, d). Quantification of the percentage of Treg, Th17, Treg17, Teff17 cells in the brains and spinal cords from three groups (b, e). Quantification of ratio of Treg/Th17 and Treg17/Teff17 in the brains and spinal cords from three groups (c, f). N = 6 in each group. Data are displayed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001