Figure 1.

Nanopore sequencing. (A) Single-stranded polynucleotide is electrophoretically driven through a nanopore that provides the only path through which ions or polynucleotides can move from the cis to the trans chamber. Residual current-vs-time signal trace from a nanopore shows clear discrimination between single bases (dGMP, dTMP, dAMP, and dCMP). (B) ssDNA threaded through a nanopore protein, and individual bases are identified, as the strand remains intact. Panels A and B are redrawn from images on Oxford Nanopore’s website.