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. 2021 Jun 24;9(7):1375. doi: 10.3390/microorganisms9071375

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(a) Dose response curves of peripheral blood mononuclear cells from four representative donors, C162–165 (n = 10), treated with Shigella four-component GMMA drug product (DP) stored at 4 °C (black inverted triangles), 25 °C (pink diamonds) or 40 °C for 3 months. The highest concentration of Shigella four-component GMMA DP was 12.5 ng/mL for donors C162, 164 and 165, and 3.125 ng/mL for donor C163. A two-way ANOVA (p > 0.05) was carried out on OD values for statistical analysis. (b) Half maximal effective dose (EC50) values from the interleukin-6 response of four donors (each donor is represented by a different colour). A one-way ANOVA test was carried out (* p < 0.05).