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. 2021 Jul 13;14(14):3909. doi: 10.3390/ma14143909

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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OE-MSCs characterization. (A) Morphology of OE-MSCs in passage one. Scale bar: 20 μm; (a,b) Morphology of OE-MSCs in passage three which was described as a spindle-like morphology. Scale bar: 10 μm; Immunocytochemistry of OE-MSCs to characterized specific neural crest and mesenchymal markers; (c) nestin; and (d) vimentin by immunofluorescence staining. Osteogenic and adipogenic differentiation of OE-MSCs, respectively; (e,f) Scale bar: 40 μm. (B) Flow cytometric assessment to characterization of OE-MSCs surface markers; including CD29 as a positive marker; CD31 as a negative marker; CD105 as a positive marker; CD90 as a positive marker; and CD34 as a negative marker.