Table 3.
IC50 values (µg/mL) for AChE, ABTS•+, and LOX assays obtained for the 32 olive leaves extracts and reference-standards studied.
| No. | SAMPLES | AChE Inhibitory Capacity | Antioxidant Capacity by ABTS | Anti-Inflammatory Capacity by LOX |
|---|---|---|---|---|
| Olive leaves extracts used as control fractions | ||||
| 01 | Global control | 408.41 ± 0.92 defg | 108.67 ± 1.88 i | 153.79 ± 0.55 hij |
| 02 | Control-20 min | 440.52 ± 24.43 efgh | 91.58 ± 0.44 hijk | 107.30 ± 2.44 bcdef |
| 03 | Control-60 min | 487.37 ± 34.20 f | 95.24 ± 1.79 hij | 187.75 ± 9.21 jk |
| 04 | Control-120 min | 405.88 ± 22.30 defg | 67.29 ± 0.61 efg | 120.64 ± 8.26 defg |
| Olive leaves extracts obtained with silicas | ||||
| 05 | SS-20 min | 436.68 ± 32.29 efgh | 62.67 ± 0.81 def | 103.30 ± 9.86 bcde |
| 06 | SS-60 min | 455.97 ± 44.87 fg | 177.16 ± 1.02 k | 159.58 ± 15.26 hi |
| 07 | SS-120 min | 300.80 ± 20.52 bc | 46.43 ± 1.32 bcd | 225.09 ± 9.18 k |
| 08 | S150–20 min | 322.88 ± 5.90 bc | 134.95 ± 0.99 jkl | 83.53 ± 7.16 ab |
| 09 | S150–60 min | 746.99 ± 35.71 hi | 258.49 ± 11.49 l | 516.51 ± 13.99 m |
| 10 | S150–120 min | 577.57 ± 25.88 g | 326.48 ± 9.09 m | 548.87 ± 68.79 n |
| 11 | S150P-20 min | 482.28 ± 34.32 f | 83.07 ± 0.31 ghij | 110.80 ± 8.93 cdef |
| 12 | S150P-60 min | 483.43 ± 28.23 f | 101.48 ± 2.16 hi | 119.24 ± 6.68 defg |
| 13 | S150P-120 min | 453.04 ± 36.93 fg | 104.72 ± 5.87 i | 171.79 ± 29.01 ij |
| 14 | S60–20 min | 805.47 ± 103.47 k | 320.67 ± 4.25 n | n.d. |
| 15 | S60–60 min | 761.14 ± 49.23 hi | 1789.61 ± 27.56 q | 201.40 ± 14.28 kl |
| 16 | S60–120 min | 790.58 ± 37.83 h | 59.30 ± 0.42 cde | n.d. |
| 17 | S60P-20 min | 788.43 ± 46.68 h | 501.75 ± 10.06 o | n.d. |
| 18 | S60P-60 min | n.d. | 391.33 ± 54.50 m | n.d. |
| 19 | S60P-120 min | 712.53 ± 49.97 h | 953.40 ± 23.52 p | 171.86 ± 11.01 ij |
| Olive leaves extracts obtained with aluminum oxide | ||||
| 20 | AO-20 min | 549.49 ± 51.36 g | 191.74 ± 0.19 k | 169.17 ± 10.65 ij |
| 21 | AO-60 min | 463.82 ± 26.86 fg | 39.99 ± 0.03 abc | 75.20 ± 9.80 a |
| 22 | AO-120 min | 449.50 ± 39.71 fgh | 79.98 ± 0.40 fghi | 319.76 ± 3.12 l |
| Olive leaves extracts obtained with zeolites | ||||
| 23 | ZeAmG-20 min | 418.40 ± 16.96 efg | 128.49 ± 0.45 j | 83.27 ± 9.23 ab |
| 24 | ZeAmG-60 min | 391.84 ± 34.09 def | 130.38 ± 2.96 jk | 192.65 ± 7.00 jk |
| 25 | ZeAmG-120 min | 417.21 ± 35.89 defg | 153.55 ± 0.04 j | 100.54 ± 3.98 abcd |
| Olive leaves extracts in the different adsorbates | ||||
| 26 | SS | 144.43 ± 29.11 a | 82.59 ± 1.08 ghij | 104.82 ± 11.40 bcdef |
| 27 | S150 | 420.78 ± 11.98 efg | 23.65 ± 0.11 a | 84.29 ± 5.82 abc |
| 28 | S150P | 270.66 ± 16.90 b | 32.68 ± 0.11 ab | 139.82 ± 11.75 ghi |
| 29 | S60 | 383.41 ± 28.25 de | 144.61 ± 0.33 jkl | 96.94 ± 7.20 abcd |
| 30 | S60P | 357.67 ± 16.71 cd | 73.00 ± 1.63 efgh | 104.34 ± 6.57 bcde |
| 31 | AO | 271.54 ± 13.39 b | 148.22 ± 3.46 jk | 131.88 ± 4.02 fgh |
| 32 | ZeAmG | 447.64 ± 43.20 fgh | 54.26 ± 0.39 cde | 129.56 ± 10.29 efgh |
| Standards | ||||
| Galantamine * | 0.40 ± 0.02 l | -- | -- | |
| Trolox * | -- | 2.50 ± 0.02 r | -- | |
| Ascorbic acid * | -- | 25.00 ± 0.30 a | -- | |
| Quercetin * | -- | -- | 19.71 ± 0.24 p | |
| Rosemary ** | 107.85 ± 8.39 a | 35.63 ± 1.14 abc | 9.82 ± 0.88 o | |
Results are expressed as the mean ± SD (n = 3). * Chemical standard; ** Reference natural extract; n.d.: no data. Different letters in the same column show significant statistical differences (p < 0.05).