Figure 3.

Prolonged moderate-high temperature affects PIN1 abundance in roots of Col-0 and act7-4 mutants. (A) PIN1-GFP and act7-4 PIN1-GFP at 23 and 29 °C. Bar = 25 µm. Arrowheads indicate the intracellular agglomeration of PIN1 (B) Quantification of GFP fluorescence intensity from (A). The images were captured using the same confocal setting. Vertical bars represent mean ± SE of the 15–20 seedlings from at least three independent experiments. Asterisks represent the statistical significance between the means of the percentage Col-0 and mutant judged by the Student’s t-test (*** p < 0.001). Five-day-old light grown seedlings were transferred to new agar plates and subjected to high-temperature treatment (29 °C) for 72 h before imaged with confocal microscopy using the same confocal settings and are representative of 15–20 seedlings obtained from three independent experiments.