Figure 4.

Prolonged moderate-high temperature diminishes PIN2 abundance drastically in act7-4 roots. (A) Cellular imaging of PIN2-GFP and act7-4 PIN2-GFP at 23 °C and 29 °C. Bar = 25 µm. Arrowheads indicate the intracellular agglomeration of PIN2 (B) Quantification of GFP fluorescence intensity from (A). The images were captured using the same confocal setting. Asterisks represent the statistical significance between the means of the percentage Col-0 and mutant judged by the Student’s t-test (*** p < 0.001). Vertical bars in the graph represent mean ± SE of the 15–20 seedlings from at least three independent experiments. Five-day-old light grown seedlings were transferred to new agar plates and subjected to high-temperature treatment (29 °C) for 72 h before imaged with confocal microscopy using the same confocal settings. Images are representative of 15–20 seedlings obtained from three independent experiments.