Figure 5.

Prolonged high temperature does not alter AUX1 abundance in Col-0 and act7-4 mutants. (A) Cellular imaging of AUX1-YFP and act7-4 AUX1-YFP at 23 and 29 °C. Bar = 25 µm. (B) Quantification of GFP fluorescence intensity from (A). Five-day-old light grown seedlings of AUX1-YFP and act7-4 AUX1-YFP were transferred to new agar plates and subjected to high-temperature treatment (29 °C) for 72 h before imaged with confocal microscopy using the same confocal settings and are representative of 15–20 seedlings obtained from three independent experiments. Bar = 25 µm.