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. 2021 Jun 24;11(7):1661. doi: 10.3390/nano11071661

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) GL261 glioma cells (in lower well) were treated with various treatments and co-cultured with RAW 264.7 Macrophages (in upper well) using 0.4 μm pored transwells. (B) GL261 cell viability in response to various treatments when co-cultured for 24 h with RAW 264.7 cells. (C) Co-culture in transwell system with a 3 μm porous membrane at the bottom of the upper chamber. Migrations of RAW 264.7 mouse macrophages towards glioma cells (chemoatxis of microphages) were noted and were labeled with VybrantTM DiD (red) in the lower chamber.