Figure 1.

HopA1_pss elicits stronger immune response than HopA1_pst in N. tabacum. Tobacco leaves were infiltrated either with buffer alone (mock) or with Agrobacterium strains expressing Myc-HopA1_pss or Myc-HopA1_pst. (a) Hypersensitive response (HR) was imaged at 24- and 48-h post-infiltration (hpi), (b) expression levels of Myc-HopA1_pss or Myc-HopA1_pst proteins determined at 24 hpi via immunoblotting with anti-Myc antibodies, (c) electrolyte leakage or conductance assay measured at indicated time points (hpi) and (d) expression of PR5 transcripts determined at 24-hpi. Ponceau S stained membrane shows comparable protein loading between samples. Values are mean ± SD (n = 10 for conductance assays, n = 3 for qRT-PCRs). Statistical analysis is according to pairwise Student’s t-test (** p < 0.01).