Table 3.
Means and standard deviations (SD) of percentages of different Phospho-AMPKα localization in swim-up-selected human sperm. Sperm were incubated for 2 or 4 h with F4-neuroprostanes (F4-NeuroPs, 7 ng). Five semen samples were analyzed. p < 0.05 is considered significant. * p < 0.05, ** p < 0.01; *** p < 0.001.
| Control (2 h) |
F4-NeuroPs (2 h) | Control (4 h) |
F4-NeuroPs (4 h) | |
|---|---|---|---|---|
| Mid-piece labeling % | 66 ± 1.8 * | 13.7 ± 1.7 ** | 17.5 ± 1.7 | 3.2 ± 1.2 |
| Dotted tail labeling % | 0.5 ± 0.5 | 64.7 ± 1 *** | 51 ± 2.6 | 15 ± 0.8 |
| Acrosomal labeling % | 1.2 ± 0.5 | 18.7 ± 2.2 | 17.5 ± 2.1 | 57.2 ± 1.7 ** |
| Any signal % | 33.5 ± 1.3 | 3.7 ± 1.2 | 14 ± 1.6 | 24.5 ± 0.6 |