. 2021 Jun 23;12(7):957. doi: 10.3390/genes12070957

Table 2.

Nuclei isolation buffers and their compositions.

Buffer	Composition
Galbraith [47]	45 mM MgCl₂, 20 mM MOPS, 30 mM sodium citrate, 0.1% (v/v) Triton X-100 (pH 7)
Otto [48]	Otto I: 100 mM citric acid, 0.5 % (v/v) Tween 20 (pH 2–3)Otto II: 400 mM Na₂PO₄ · 12H₂O (pH 8–9)
Modified Otto (this paper)	Otto I: 100 mM citric acid, 0.5 % (v/v) Tween 20 (pH 2–3)Otto II: 400 mM Na₂PO₄ · 12H₂O (pH 8–9), 15 mM β-mercaptoethanol, 1% PVP-40
Partec [49]	100 mM Tris, 2.5 mM MgCl₂ · 6H₂O, 85 mM NaCl, 0.1% (v/v) Triton X-100 (pH 7,0)
Modified Partec (this paper)	100 mM Tris, 2.5 mM MgCl₂ · 6H₂O, 85 mM NaCl, 0.1% (v/v) Triton X-100 (pH 7.0), 15 mM β-mercaptoethanol, 1% PVP-40