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. 2021 Jul 14;9(7):1504. doi: 10.3390/microorganisms9071504

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Panel of the gathered images. (1A,C) Organisms stained by the probe SELE appear as densely packed groups in the cervical portion of the subgingival biofilm. (1B) Higher magnification shows a crescent-shaped morphology. (1C,D) EUB338_FITC (Fluorescein isothiocyanate) (green), SELE_Cy3 (Cyanine 3) (bright orange) and FUSO_Cy5 (Cyanine 5) (magenta). (1D) Higher magnification, with EUB338_FITC filter removed for better interpretation. Bars indicate 10 µm. (2) FISH (Fluorescence in situ hybridization) performed on a gingival biopsy. (2A) EUB338_FITC (green), SELE_Cy3 (orange), and eukaryotic cell nuclei stained with DAPI (4′, 6-diamino-2-phenylindole) (blue). (2B) Higher magnification took with the Cy3 filter set only. Bars indicate 10 µm. All images were reprinted and adapted with the publisher’s permission.