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. 2021 Jul 19;22(14):7696. doi: 10.3390/ijms22147696

Figure 1.

Figure 1

Single-cell transcriptome analysis of the decidualized mouse uterus on gestational day 8. (A) A flowchart overview of this study. IIS, inter-implantation site (undecidualized uterus, served as control); IS, implantation site (decidualized uterus). (B) A photograph of mouse uterus on gestational day 8. The positions of embryo implantation sites were marked with an arrow. (C) Cell viability analysis of single-cell suspension. Cells were stained with the AO/PI solution. Cells in green were live and cells in yellow were dead. A representative photo was provided, and a bar plot was shown on the right. n = 3; * p < 0.05. (D,E) Single-cell RNA-seq data pre-processing and quality control. Cells with detected genes of fewer than 200 or more than 6000 were removed. Only cells with total mitochondrial gene expression below 25% were kept. (F) Scatter plots showing the correlation between single-cell RNA-seq and bulk RNA-seq. For single-cell RNA-seq data, gene expression levels were averaged and normalized as transcript per million (TPM). For bulk RNA-seq data, gene expression levels were measured as fragments per kilobase per million (FPKM).