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. 2021 Jul 7;22(14):7305. doi: 10.3390/ijms22147305

Figure 5.

Figure 5

Fluorescence detection in 4 days old A. thaliana pDR5::GFP transgenic line (auxin responsive reporter) grown for 48 h in untreated agar medium ((a) (RAM) and (c) (elongation zone)) or 100 μM of coumarin enriched agar medium ((b) (RAM) and (d) (elongation zone)). Scale bars 34 μm. N = 20. (e) Integrated optical density (IOD) expressed as arbitrary units (AU) of fluorescence intensity measured in the root tip and the MZ (maturation zone) of Arabidosis treated for 48 h with 0 or 100 μM of coumarin (N = 10); (f) auxin content in Arabidosis roots treated for 48 h with coumarin 0 (C) or 100 (CUM) μM. Data are presented as mean ± standard deviation (SD). Statistical analysis was performed using the Student’s t-test with * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. N = 4.