Figure 1.

Francisella sp. detection in surface water samples collected during both campaigns. For each site, DNA was extracted from water, and three q-PCR tests were performed: ISFtu2-qPCR for Francisella sp., Tul4-qPCR for F. tularensis/F. novicida, and Type B-qPCR for F. tularensis subsp. holarctica. Circle: water sampling site. White circle: sample with no positive qPCR. Green circle: sample with positive ISFtu2-qPCR in the first campaign, or the second campaign, or both, and negative Tul4 and Type B-qPCR. Orange circle: sample with positive ISFtu2 and Tul4-qPCR in the first campaign, or the second campaign, or both, and negative Type B-qPCR. Red circle: sample with positive ISFtu2, Tul4, and Type B-qPCR in the first campaign, or the second campaign, or both. Disks indicate sites where water samples were collected during the two campaigns.