Table 2.
The qPCR control results.
| DNA Extracts | ISFtu2-qPCR (Ct) | Tul4-qPCR (Ct) | Type B-qPCR (Ct) |
|---|---|---|---|
| Serratia marcescens CIP 103551 * | 37 | Negative | Negative |
| Streptococcus equi ATCC 43079 * | 37 | Negative | Negative |
| F. noatunensis LMG 23800 * | 36 | Negative | Negative |
| F. philomiragia ATCC 25015 * | 36 | Negative | Negative |
| F. novicida U112 * | 12 | 16 | Negative |
| Clinical strain Ft92 of F. tularensis subsp. holarctica * | 12 | 15 | 16 |
| LVS-spiked water 0.1 CFU/l | 38 | Negative | Negative |
| LVS-spiked water 1 CFU/l | 36 | Negative | Negative |
| LVS-spiked water 10 CFU/l | 36 | 37 | Negative |
| LVS-spiked water 100 CFU/l | 34 | 38 | 38 |
| LVS-spiked water 1000 CFU/l | 27 | 33 | 33 |
| LVS-spiked water 10,000 CFU/l | 25 | 30 | 30 |
* 50 ng of DNA introduced in the PCR mix.