Figure 2.

Ziziphus nummularia induces apoptosis in Capan-2 cancer cells. (a) Cells were treated for 48 h with or without the indicated concentrations of ZNE. The expression levels of procaspase-3 were determined by Western blotting and β-actin used as loading control. The blot shown is representative of three independent experiments. (b) Caspase 3/7 activity was assessed 48 h after treatment of Capan-2 cells with ZNE (100 and 200 μg/mL) or ethanol (control) cells. The relative caspase 3/7 activity was normalized to the number of viable cells per well and is expressed as fold of induction compared to the untreated control cells. * denotes p < 0.05.