Figure 6.

Ziziphus nummularia inhibits the adhesion of Capan-2 cells to collagen and downregulates α₂ integrin. (a) Capan-2 cells were treated overnight with or without the indicated concentrations of ZNE and then seeded onto collagen-coated wells and allowed to adhere for 10 min as described under Materials and Methods. Adhesion was assessed by MTT assay and determined as a percentage of the corresponding vehicle-treated control value. Data represent the mean ± SEM (n = 3 replicates) from three independent experiments. ** denotes p < 0.01. (b) Capan-2 cells were incubated for 48 h with or without the indicated concentrations of ZNE, and whole cell extracts were subjected to Western blotting analysis for integrin α₂ expression using β-actin as loading control. Data represent the mean ± SEM from three independent experiments. * denotes p < 0.05.