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. 2021 Jul 15;13(14):3544. doi: 10.3390/cancers13143544

Figure 5.

Figure 5

EGFR amplification was associated with acrolein-induced DNA damages in OSCC patients. (A) Gene copy number of EGFR was analyzed in buccal DNA of control subjects and tumor tissues and normal buccal DNA of 18 OSCC patients using quantitative real-time PCR. Data were expressed relative to the LINE1 gene. Student’s t-tests were used to determine statistical significance, and two-tailed p-values are shown. *** p < 0.005 compared with control subjects, # p < 0.05 compared with normal buccal DNA of OSCC patients. (B) Gene copy number of EGFR in tumor tissues and counterpart normal buccal DNA of 18 OSCC patients. (C) Correlation between EGFR gene copy number and relative acrolein levels in 18 OSCC patients was evaluated by Pearson correlation analysis (p < 0.001). Relative acrolein-induced DNA (Acr-dG) levels in tumor tissues and counterpart normal buccal DNA of 18 OSCC patients were analyzed using slot blot analysis. Closed circle, tumor samples; open circle, normal buccal DNA.