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Primer oligos designed for this study.
| Primer Name | Sequence (5′-3′) | Melting Temperature (°C) | Fixed or Repeat |
|---|---|---|---|
| Forward | |||
| F1 | CGAACGCTTTCTCAAACCAC | 60.81 | Fixed |
| F2 | CCTTTTCTCTCACACACGCTAC | 59.05 | Fixed |
| R1F | ATGAGGCCCAAGCATTTCTG | 62.43 | Repeat |
| Reverse | |||
| R1 | AGAAATGCTTGGGCCTCATT | 60.96 | Repeat |
| R1R | CCACTTCGACAGCTTTCTCC | 59.99 | Fixed |
| R2R | AGGCCCAAGCATTTCTGC | 61.30 | Fixed |
