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. 2021 Jul 12;10(7):1762. doi: 10.3390/cells10071762

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Putative interactions of SARS-CoV-2 v-miRNA-N were found in host genes related to inflammasome activation. (A) A summary of the binding sites of v-miRNA-N-28612, v-miRNA-N-29094, and v-miRNA-N-29443 in the 3′UTR sites of IL-1β and CASP 1 gene. (B) and (C) show the binding sites for v-miRNA-N-29094 and v-miRNA-N-29443 in the 3′UTR regions of IL-1β and CASP 1 gene, which are analyzed using Sfold STarMir (https://sfold.wadsworth.org/cgi-bin/starmirtest2.pl) (accessed on 25 May 2021). The seed sequences of v-miRNA-N species are shown in red. (D) Western blot results show the expression of IL-1β, NLRP3, and caspase 1 (Casp1) proteins in PBMCs transfected with negative control (Ctrl; leftside) or v-miRNA-N (v-mi-N; rightside) synthetic mimics. n = 6 (obtained from biological replicates); ** p < 0.01. CASP 1, caspase 1; IL-1β, interleukin one beta; NLRP3, nucleotide oligomerization domain-like receptors family pyrin domain containing 3; v-miRNA-N, viral microRNA nucleocapsid.