Table 1.

Strains and plasmids used in this work. The superscript “R” after antibiotics denotes resistance.

Bacterial Species	Designation in This Work	Description	Reference
L. monocytogenes	WT	wild-type strain Scott A; WGS accession number at NCBI: CM001159	[17]
	WT/pIMK2	WT with pIMK2 integrated, KmR
	yvcK::Himar1	Transposon insertion in yvcK, EmR
	yvcK/pIMK3	yvcK::Himar1 with pIMK3 integrated, KmR EmR
	yvcK/pIMK3-yvcK	yvcK::Himar1 with pIMK3-yvcK integrated, KmR EmR
	yvcK/pIMK3-glmU	yvcK::Himar1 with pIMK3-glmU integrated, KmR EmR
	yvcK/pIMK2-glmM	yvcK::Himar1 with pIMK2-glmM integrated, KmR EmR
	yvcK/pIMK2-glmS	yvcK::Himar1 with pIMK2-glmS integrated, KmR EmR
	yvcK::Himar1 ΔnagR	In-frame deletion of nagR in yvcK::Himar1 strain, EmR
	yvcK::Himar1 ΔnagR/pIMK2	yvcK::Himar1 ΔnagR with pIMK2 integrated, KmR EmR
	yvcK/pIMK2-nagR	yvcK::Himar1 with pIMK2-nagR (WT allele) integrated, KmR EmR
	yvcK/pIMK2-nagRM	yvcK::Himar1 with pIMK2-nagR (mutated allele from M 2.2 suppression mutant) integrated, KmR EmR
E. coli	S17-1 λpir	Donor strain for plasmid conjugation	[19]
	DH5-α	Host strain for plasmid constructs	[18]
Plasmids	Description		Reference
pIMK2	Site-specific listerial integrative vector, Phelp constitutive promoter, 6.2 kb, KmR		[21]
pIMK3	Site-specific listerial integrative vector, Phelp IPTG inducible promoter, 7.5 kb, KmR		[21]
pIMK3-yvcK	pIMK3 with yvcK gene (locus tag: LMOSA_4390) from Scott A
pIMK3-glmU	pIMK3 with glmU gene (locus tag: LMOSA_10910) from Scott A
pIMK2-glmM	pIMK2 with glmM gene (locus tag: LMOSA_1060) from Scott A
pIMK2-glmS	pIMK2 with glmS gene (locus tag: LMOSA_16310) from Scott A
pIMK2-nagR	pIMK2 with nagR gene (locus tag: LMOSA_18480) from Scott A
pIMK2-nagRM	pIMK2 with mutated nagRM gene from M 2.2 suppression mutant strain
pKSV7-oriT	Temperature-sensitive shuttle vector for making gene deletion in L. monocytogenes, 6742 bp, AmpR, CmR		[22,23]
pKSV7-oriT--∆nagR	pKSV7-oriT with 1 kb flanking fragments upstream and downstream of nagR inserted