Table 5.

The features and description of the positive selection sites detected in the mitochondrial PCGs of Heptageniidae species.

Genes	Positive Selection Sites	Amino Acids		BEB Value	Feature Key *	Description
		Foreground	Background
COX1	408	N	Q/K	0.988 *	Domain	COX1
	475	A	S	0.979 *	Domain	COX1
Cyt b	62	T	L	0.977 *	Domain	CYTB_NTER
	358	T/I	V/T/I	0.954 *	Transmembrane	Helical
ND1	173	Y	E/K/Q	0.989 *	/	/
	189	S	A/T	0.983 *	Transmembrane	Helical
ND2	148	S/G	P/S	0.995 **	Transmembrane	Helical
	153	T/S	F	0.983 *	Transmembrane	Helical
	197	N	S/P/N	0.979 *	Domain	Proton_antipo_M
	318	G/N	S/L/T/A	0.983 *	/	/
	341	S	S/P/T/L	0.991 **	/	/
ND3	85	Q	A/N/S/T	0.970 *	/	/
ND4	183	S	G/S/K	0.962 *	Transmembrane	Helical
	189	G	M/L	0.986 *	Transmembrane	Helical
ND5	25	T	L	0.971 *	Transmembrane	Helical
	29	L/A	S	0.964 *	Transmembrane	Helical
	179	E/H	S/G/T	0.969 *	Transmembrane	Helical
	337	S	S/N/I/T	0.984 *	Domain	Proton_antipo_M
	468	L	S/V/I/A	0.989 *	Transmembrane	Helical
	490	F	N/H/Q/G/S	0.978 *	Domain	NADH5_C
ND6	3	T	L/F/M	0.976 *	/	/
	12	L	T/I	0.996 **	Transmembrane	Helical
	28	I/V	C/S/I/V	0.985 *	Transmembrane	Helical
	110	S	E/D	0.996 **	/	/
	111	D	Q	0.984 *	/	/
	125	P	N/T/I/G	0.993 **	Transmembrane	Helical
	158	N	Q/N	0.981 *	Transmembrane	Helical

Note: * and ** indicate BEB values > 0.95 and > 0.99, respectively.