Table 1.

TCR/pMHCI dissociation constants and kinetics for agonists of the MEL5 TCR

MHCI	Epitope	kon (M−1s−1)	koff (s−1)	KD (μM)
HLA-A2	ELTGIGILTV (3T)	∼1.2 × 104	∼1	82 ± 4
HLA-A2	ELAGIGILTV (ELA)	∼4.4 × 104	∼0.75	17 ± 1
HLA-A2	FATGIGIITV (FAT)	∼1.2 × 105	0.36	3 ± 1
HLA-A2	ILAGIGILTV (1I)	∼1.3 × 105	∼1.3	10.2 ± 0.7*

^*Surface plasmon resonance data are shown in SI Appendix, Fig. S6A.