Table 1.
Analysis of spontaneous rat behavior. Data presented as path length; locomotion, resting, and rearing times; number of all rearings, side wall rearings (supported), and free rearings (unsupported) measured in actometers, showed as mean raw values ± SEM. Analysis was performed at time-points 3, 5, 13, 27 days after brain surgery. Animals were kept on ketogenic diet for 3 weeks in advance. p value ≤ 0.05 was set as significance threshold and marked as * vs. normal diet + sham (NS group), L vs. normal diet + 6-OHDA lesion (NL group), K vs. ketogenic diet + sham (KS group), KL stands for ketogenic diet + 6-OHDA lesion group. Repeated measure ANOVA with LSD post hoc test for analysis in time and 3-way ANOVA with LSD post hoc test for analysis of total values were performed. Number of animals in groups was: for 3 days, n = 19–26; for 5 days, n = 8–12; for 13 days, n = 9–16; for 27 days, n = 8–14.
| Time | Group | Path | Locomotion | Resting | Rearing | Rearing | Rearing | Rearing |
|---|---|---|---|---|---|---|---|---|
| Length | Time | Time | Time | Total | Supported | Free | ||
| (cm) | (% of Time) | (s) | (s) | (Number) | (Number) | (Number) | ||
| 3 DAYS | NS | 222.1 ± 11.7 | 13.2 ± 0.8 | 9558 ± 833 | 91.1 ± 4.4 | 10.0 ± 0.8 | 4.9 ± 0.5 | 5.1 ± 0.4 |
| NL | 111.6 * ± 15.8 | 4.9* ± 1.0 | 12,643 * ± 885 | 55.4 * ± 8.3 | 3.4 * ± 0.6 | 1.9 * ± 0.3 | 1.6 * ± 0.3 | |
| KS | 243.0 L ± 16.5 | 14.7 L ± 1.1 | 10,881 ± 731 | 61.9 * ± 3.9 | 8.5 L ± 0.9 | 4.8 L ± 0.5 | 3.6 *,L ± 0.4 | |
| KL | 63.8 *LK ± 13.2 | 2.6 *,K ± 0.9 | 16,395 *,L,K ± 1957 | 23.9 *,L,K ± 6.0 | 0.8 *,L,K ± 0.3 | 0.4 *,L,K ± 0.1 | 0.3 *,L,K ± 0.1 | |
| 5 DAYS | NS | 275.6 ± 17.6 | 16.2 ± 1.3 | 6334 ± 689 | 114.3 ± 8.9 | 12.5 ± 0.9 | 6.8 ± 0.7 | 5.6 ± 0.5 |
| NL | 157.0 * ± 33.6 | 7.8 * ± 2.4 | 11,627 * ± 1009 | 110.7 ± 24.3 | 4.8 * ± 1.5 | 2.5 * ± 0.9 | 2.3 * ± 0.6 | |
| KS | 346.7 *,L ± 35.1 | 20.8 *,L ± 2.2 | 6459 L ± 667 | 79.8 ± 8.1 | 10.6 L ± 1.6 | 6.4 L ± 1.0 | 3.7 *,L ± 0.4 | |
| KL | 116.5 *,K ± 19.7 | 4.5 *,K ± 1.0 | 13,814 *,K ± 1781 | 56.7 *,L,K ± 12.7 | 2.9 *,K ± 0.9 | 1.4 *,K ± 0.4 | 1.5 *,K ± 0.6 | |
| 13 DAYS | NS | 246.7 ± 13.8 | 13.4 ± 1.0 | 6871 ± 526 | 125.4 ± 5.4 | 10.9 ± 0.9 | 5.9 ± 0.6 | 5.0 ± 0.3 |
| NL | 213.4 ± 25.3 | 10.2 ± 1.8 | 6215 ± 692 | 168.1 * ± 17.1 | 10.7 ± 1.1 | 5.4 ± 0.8 | 4.3 ± 0.7 | |
| KS | 330.1 *,K ± 22.5 | 20.3 *,L ± 1.5 | 5285 ± 1044 | 101.7 L ± 9.0 | 10.7 ± 1.0 | 6.9 ± 0.8 | 3.6 *,L ± 0.3 | |
| KL | 248.8 K ± 36.6 | 12.32 K ± 2.8 | 7575 K ± 1194 | 119.8 L ± 11.5 | 7.2 *,L,K ± 1.2 | 4.4 K ± 0.7 | 2.3 *,L ± 0.6 | |
| 27 DAYS | NS | 294.2 ± 19.2 | 17.2 ± 1.3 | 4868 ± 617 | 130.6 ± 8.0 | 14.3 ± 0.8 | 8.1 ± 0.6 | 6.2 ± 0.3 |
| NL | 264.3 ± 31.7 | 13.7 ± 2.2 | 7705 * ± 1317 | 148.2 ± 11.4 | 12.2 ± 1.3 | 6.8 ± 0.8 | 5.4 ± 0.7 | |
| KS | 306.8 ± 23.6 | 18.4 ± 1.6 | 6616 ± 971 | 99.3 *,L ± 6.6 | 9.6 * ± 0.9 | 5.6 * ± 0.7 | 4.0 * ± 0.3 | |
| KL | 346.3 L ± 45.1 | 18.9 ± 2.9 | 8360 * ± 1313 | 112.6 L ± 11.7 | 8.5 *,L ± 1.7 | 4.9 * ± 0.9 | 3.6 *,L ± 0.8 |