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. 2021 Jul 8;22(14):7342. doi: 10.3390/ijms22147342

Figure 4.

Figure 4

Scleroderma (A–E) and basophilic degeneration of the collagen (F,G). (A,B) Double immunochemistry for CD34 (brown) and αSMA (red). Hematoxylin counterstain. (C,D) Double immunofluorescence labelling for CD34 (green) and Collagen I (red). DAPI counterstain. (E) (1,2,3): Confocal microscopy, frontal view. Immunofluorescence labelling for CD34 (green) and αSMA (red). (F,G) Ultrathin sections. Uranyl acetate and lead citrate. (A–E) Stromal cells expressing CD34 (brown in (A) and green in C,E) or αSMA (red) in scleroderma. Note the expression of CD34 around Collagen I (red) in C and the regional absence of CD34 expression in (D). Stromal cells co-expressing CD34 (green) and αSMA (red) are observed in (E) (1,2,3). (F,G) Ultrastructural images of telopodes (arrows) closely associated with several degenerative dermal fibers (df) in basophilic degeneration of the collagen. Bar: (A,B) 25 µm, (C–G) 2.5 µm.