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. 2021 Jul 2;10(7):1664. doi: 10.3390/cells10071664

Figure 1.

Figure 1

The 660-nanometer LED Irradiation Schedule. (A) Representative image of LED irradiation. Graph showing that the LED irradiation was delivered at 660 nm; (B) Schema of the astrocyte culturing method and experimental schedule. From DIV0 to DIV6, cells were incubated in DMEM+ (DMEM, FBS, P/S [P = 5000 U/mL, S = 5000 μg/mL]). On DIV6, cells were subcultured for isolation of astrocytes. Cells were incubated in NB+ medium (Neurobasal, B27, GlutaMAX, P/S (P = 5000 U/mL, S = 5000 μg/mL)). The LED irradiation protocol was divided into the following two methods: irradiation on DIV7 only, and irradiation on both DIV7 and DIV10. Each colored circle denotes the sampling time for several experiments. The red circle indicates verification for cell differentiation evidence, and the blue and green circles indicate cell proliferation and ROS assay, respectively.