Table 2.
Demonstration of Presented Cell Types Depends on Protein Expression. Glial fibrillary acidic protein: GFAP; aldehyde dehydrogenase 1 family member L1: aldh1L1; octamer-binding transcription factor 4: Oct4U: Mann–Whitney U-test; t: Unpaired t-test; (* p < 0.05; ** p < 0.01).
| Cell Type | Protein Expression | Ctrl vs. 660 nm | ||
|---|---|---|---|---|
| Mean ± SEM (# of Cells) | p-Value | U or t | ||
| Pluripotent stem cell | Oct4+/GFAP− | 16.36 ± 3.026 vs. 22.67 ± 2.826 (35) | 0.0595 | 452 |
| Oct4+/aldh1L1− | 2.166 ± 0.6865 vs. 2.735 ± 0.6442 (36) | 0.1542 | 531.5 | |
| Neural stem cell | Nestin+/GFAP− | 6.725 ± 2.072 vs. 17.7 ± 2.648 (38) | 0.0057 ** | 462.5 |
| Nestin+/aldh1L1− | 0.1517 ± 0.1425 vs. 2.446 ± 1.3 (39) | 0.0335 * | 640.5 | |
| Developmental astrocytes | GFAP+/Oct4− | 2.5 ± 0.6376 vs. 3.343 ± 0.5668 (35) | 0.1681 | 495 |
| GFAP+/Nestin− | 1.782 ± 0.2909 vs. 1.497 ± 0.2142 (39) | 0.8988 | 709.5 | |
| Maturity astrocytes | aldh1L1+/Oct4− | 8.484 ± 1.474 vs. 9.874 ± 1.694 (36) | 0.1609 | 523 |
| aldh1L1+/Nestin− | 5.219 ± 0.9469 vs. 9.785 ± 1.786 (39) | 0.0617 | 573.5 | |
| Immature astrocytes with ability to differentiate to other specific cell types | GFAP+/Oct4+ | 58.78 ± 2.59 vs. 49.98 ± 2.88 (35) | 0.0240 * | 421 |
| GFAP+/Nestin+ | 56.76 ± 2.474 vs. 52.57 ± 3.059 (38) | 0.2901 | 1.065 | |
| Mature astrocytes with ability to differentiate to other specific cell types | aldh1L1+/Oct4+ | 74.23 ± 1.921 vs. 71.37 ± 2.345 (36) | 0.5177 | 590 |
| aldh1L1+/Nestin+ | 70.99 ± 1.686 vs. 66.17 ± 2.435 (39) | 0.1907 | 629 | |