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. 2021 Jul 16;22(14):7640. doi: 10.3390/ijms22147640

Figure 1.

Figure 1

Cell viability, degranulation, and chemokine expression in human intestinal mast cells (hiMC) following treatment with resveratrol. Evaluation of cytotoxic effects of resveratrol on hiMC (A). Cells/well were incubated with 1, 5, 10, 25, 50, and 100 μM of resveratrol and the corresponding DMSO control for 24 h. After incubation, living cells in percent of the DMSO control is shown (n = 3). Release of β-hexosaminidase by hiMC (B) (n = 6) and mRNA expression of Cxcl8 (C) (n = 4), Ccl2 (D) (n = 6), Ccl3 (E) (n = 6), and Ccl4 (F) (n = 6). Cells were incubated with 1–100 μM of resveratrol for 60 min prior to stimulation by FcεRI crosslinking using 100 ng/mL of monoclonal antibody (mAb) 22E7 for 90 min. Controls were treated with the corresponding concentration of the vehicle DMSO. Values are mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 compared to induced control treated with DMSO.