Figure 3.

Phosphorylation of signal transducer and activator of transcription 3 (STAT3) and extracellular signal-regulated kinase 1/2 (ERK1/2) in hiMC following treatment with stattic, nobiletin, and resveratrol. Western blot analyses of (A) phosphorylated STAT3 (n = 5) and (B) phosphorylated ERK1/2 (n = 4) in hiMC. HiMCs were treated with 60 μM of stattic (S), 45 μM of nobiletin (N), 50 μM of resveratrol (R), or corresponding concentrations of the vehicle DMSO (control) before stimulation by FcεRI crosslinking using 100 ng/mL of mAb 22E7 for 5 min. Representative pictures and densitometric analyses in relation to stimulated control are shown. Values are mean ± SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.