Figure 4.

Phosphorylation of STAT3 and ERK1/2 in nuclear and mitochondrial fractions of hiMC following treatment with resveratrol. Western blot analyses of two independent experiments of (A) phosphorylated ERK1/2 and (B) phosphorylated STAT3 after subcellular fractionation in pure mitochondria (Mito), cytosol fraction (Cyto), and crude nuclear fraction (Nuc), respectively. HiMCs were treated with 50 μM of resveratrol (R), or corresponding concentrations of the vehicle DMSO (control), and stimulated by FcεRI crosslinking with 100 ng/mL of mAb 22E7 for 5 min. Exemplary pictures and densitometric analyses in relation to stimulated control for Western blots of phosphorylated ERK1/2 and total ERK1/2, and phosphorylated STAT3 and total STAT3, are shown. Values are mean ± SEM (n = 2).