Figure 6.

Smad5-p75NTR signaling regulates BMP7-promoted human LN18 glioblastoma cell transmigration and migration. Human LN18 glioblastoma cells were transfected with control-, Smad5- or p75NTR-specific siRNA and then kept as the controls or treated with BMP7 (50 ng/mL) for 24 h. (A–C) The transmigration capabilities (A), p75NTR expression (B), and migration capabilities (C) of human LN18 glioblastoma cells were determined by the transwell assay, Western blot, and wound-healing assay, respectively. (A) *, p < 0.05 vs. siCL-CL group. #, p < 0.05 vs. siCL-BMP7 group. (B) *, p < 0.05 vs. siCL group. (C) *, p < 0.05 vs. 0 h group. #, p < 0.05 vs. siCL-24 h group. Scale bars in (A,C) are 100 μm.