. 2021 Jul 8;10(7):1587. doi: 10.3390/foods10071587

Table 2.

Validation parameters of the SLE-MSPE-UHPLC-QqQ-MS/MS method for the determination of six opium alkaloids in poppy seeds samples.

Analytes	Linear Range (µg/L)	Matrix-Matched Calibration (R²)	Cm	ME	MDLn (µg/kg)	MQL (µg/kg)	Accuracy		Precision
Analytes	Linear Range (µg/L)	Matrix-Matched Calibration (R²)	Cm	ME	MDLn (µg/kg)	MQL (µg/kg)	Recovery (% ± SD)	Mean Recovery (% ± SD)	Intra-Day Precision (RSD %)	Inter-Day Precision (RSD %)
Morphine	0.01–5000	y = 3269x + 32,452 (0.999)	92	64	0.07	0.24	50 ± 1 ^a	46 ± 2	4 ^a	11 ^a
Morphine	0.01–5000	y = 3269x + 32,452 (0.999)	92	64	0.07	0.24	42 ± 2 ^b	46 ± 2	2 ^b	5 ^b
Codeine	0.01–5000	y = 2554x + 29,642 (0.999)	96	86	0.07	0.24	64 ± 2 ^a	68 ± 4	3 ^a	8 ^a
Codeine	0.01–5000	y = 2554x + 29,642 (0.999)	96	86	0.07	0.24	71 ± 6 ^b	68 ± 4	2 ^b	4 ^b
Thebaine	0.1–5000	y = 10,093x + 485,953 (0.999)	96	109	0.72	2.40	72 ± 3 ^a	74 ± 4	5 ^a	7 ^a
Thebaine	0.1–5000	y = 10,093x + 485,953 (0.999)	96	109	0.72	2.40	76 ± 4 ^b	74 ± 4	0 ^b	4 ^b
Papaverine	0.01–5000	y = 33,441x + 2,814,320 (0.999)	92	80	0.07	0.24	116 ± 5 ^a	109 ± 3	5 ^a	9 ^a
Papaverine	0.01–5000	y = 33,441x + 2,814,320 (0.999)	92	80	0.07	0.24	101 ± 1 ^b	109 ± 3	2 ^b	7 ^b
Noscapine	0.01–5000	y = 42,914x + 1,631,091 (0.999)	94	65	0.07	0.24	109 ± 1 ^a	103 ± 1	4 ^a	10 ^a
Noscapine	0.01–5000	y = 42,914x + 1,631,091 (0.999)	94	65	0.07	0.24	97 ± 1 ^b	103 ± 1	3 ^b	8 ^b
Oripavine	10–5000	y = 1080x − 31,137 (0.999)	94	31	72.07	240	51 ± 3 ^a	52 ± 3	3 ^a	5 ^a
Oripavine	10–5000	y = 1080x − 31,137 (0.999)	94	31	72.07	240	53 ± 3 ^b	52 ± 3	3 ^b	5 ^b

Cm: linearity coefficient calculated by (1-(SD/average slope)) × 100, where SD is the standard deviation of the calibration slopes obtained on different days; ME: matrix effect calculated by dividing the purified matrix slope by the solvent slope. MDL: method detection limit. MQL: method quantification limit estimated as 3 and 10 times the signal/noise ratio, respectively. Accuracy (mean recovery obtained from six samples, n = 6) and precision were obtained by spiking samples at two known concentration levels: ^a low spiked level (0.1 mg/kg) and ^b high spiked level (1 mg/kg). Intra-day precision: six consecutive injections (n = 6) on the same day; Inter-day precision: three replicate samples injected in triplicate throughout three different days (n = 9).