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. 2021 Jul 11;10(7):1754. doi: 10.3390/cells10071754

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The expression of p53 (Panel (A)), p21 (WAF1/CIP1) (Panel (B)), and BAX (Panel (C)) was evaluated in human Adipose-derived stem cells (hADSCs) cultured with 4d-MCF-7-EM (4d – red bars), 7d-MCF-7-EM (7d – green bars), or 10d-MCF-7-EM (10d – blue bars). The mRNA levels for each gene were normalized to Glyceraldehyde-3-Phosphate-Dehydrogenase (GAPDH) and expressed as fold of change (2^−ΔΔCt) of the mRNA levels observed in untreated control hADSCs (Ctrl - Grey bar) defined as 1 (mean ± SD; n = 6). Data are expressed as mean ± SD referred to the control (* p ≤ 0.05).