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. 2021 Jul 11;10(7):1754. doi: 10.3390/cells10071754

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expression of epigenetic modulating genes. The expression of SIRT1 (Panel (A)) and DNMT1 (Panel (B)) was evaluated in hADSCs cultured in the presence of 4d-MCF-7-EM (4d - red bars), 7d-MCF-7-EM (7d - green bars), or 10d-MCF-7-EM (10d - blue bars). The mRNA levels for each gene were normalized to Glyceraldehyde-3-Phosphate-Dehydrogenase (GAPDH) and expressed as fold of change (2^−ΔΔCt) of the mRNA levels observed in untreated control hADSCs (Ctrl - grey bar) defined as 1 (mean ± SD; n = 6). Data are expressed as mean ± SD referred to the control (* p ≤ 0.05), (** p ≤ 0.01).