Figure 3.

Lrp6^IEC-KO mice are more sensitive to DSS-induced colitis. Ten- to twelve-week-old Lrp6^IEC-KO and control mice were treated with 1.75–2% DSS in water for 7 days. (A) Body weight was measured every day (n = 18). (B) Disease activity index of Lrp6^IEC-KO and control mice was calculated by scoring stool softness, occult fecal blood, rectal bleeding and colon rigidity at euthanasia (n ≥ 15). (C,D) H&E staining was performed on Lrp6^IEC-KO and control colon tissues to score damage (C) according to the extent of mucosal architecture destruction, immune cell infiltration, goblet cell depletion, muscle thickening and crypt abscesses (n ≥ 15). Bars: 200 µm. Data are expressed as mean ± SEM. Mann–Whitney U test, * p ≤ 0.05.