Table 1.

Primer pairs, annealing temperatures, and expected length of amplicons.

Genetic Marker	Primers (Forward, Reverse)	Nucleotide Sequence (5′–3′)	Annealing Temperature	Product Size	Reference
18S rDNA	Bc_F1	CGTAGTTGTATTTTTGCGT	50 °C	≈430 bp	[33]
	GR2	CCAAAGACTTTGATTTCTCTC
	Bc_F2	CATTTGGTTGGTTATTTCGTTTT	53 °C	376 bp
	Bc_R1	GTTCCTGAAGGGGTCAAAAA
18S rDNA	BT1F	GGTTGATCCTGCCAGTAGT	65 °C–55 °C	≈1730 bp	Modified after [8]
	BT outer R	GGAAACCTTGTTACGACTTCTC
	Piro0F2	GCCAGTAGTCATATGCTTGTCTTA	65 °C–55 °C	≈1670 bp
	BT inner R	TTCTCCTTCCTTTAAGTGATAAG
Cytb	Bc_cytb_F1	TGGTCWTGGTATTCWGGAATG	50 °C	≈700 bp	[34]
	Bc_cytb_R1	AAGMYARTCTYCCTAAACATCC
	Bc_cytb_F2	RATKAGYTAYTGGGGAGC	48 °C	≈580 bp
	Bc_cytb_R2	GCTGGWATCATWGGTATAC
COI	Bab_For1	ATWGGATTYTATATGAGTAT	45 °C	1250 bp	[34]
	Bab_Rev1	ATAATCWGGWATYCTCCTTGG
	Bab_For2	TCTCTWCATGGWTTAATTATGATAT	49 °C	980 bp
	Bab_Rev2	TAGCTCCAATTGAHARWACAAAGTG